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Although fluorescent probes and assays, which use energy transfer (ET) for monitoring DNA reactions, have Designs and Protocols Selection of Fluorophore and Quencher Pairs for Fluorescent Nucleic Acid Hybridization Probes.
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Introduction to qPCR

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DNA assay based on Nanoceria as Fluorescence Quenchers (NanoCeracQ DNA assay) | Scientific Reports

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These probes signal detection of their targets by changing either the intensity or the color of their fluorescence. However, in spite of this explosive growth in the field, there are no manuals summarizing different p- tocols and fluorescent probe designs. See details. Buy It Now.

Designs and Protocols

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About this product Product Information Fluorescent nucleic acid probes, which use energy transfer, include such constructs as molecular beacons, molecular break lights, Scorpion primers, TaqMan probes, and others. Not surpr- ingly, these luminous, multicolored probes carry more flashy names than their counterparts in the other fields of molecular biology.


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In recent years, fluor- cent probes and assays, which make use of energy transfer, have multiplied at a high rate and have found numerous applications. Oligonucleotides with one or several chromophore tags can form fluor- cent probes capable of energy transfer.

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Energy transport within the probe can occur via the resonance energy transfer mechanism, also called Forster tra- fer, or by non-Forster transfer mechanisms. Although the probes using Forster transfer were developed and used first, the later non-Forster-based probes, such as molecular beacons, now represent an attractive and widely used option.

RetailJumbo 3. The first comprehensive treatment of energy transfer ET nucleic acid probes. Hands-on experts thoroughly describe all the major probes, both fluorescence resonance energy transfer FRET -based and non-FRET-based, and provide a complete set of techniques to monitor DNA and RNA reactions, including hybridization, amplification, cleavage, folding, and associations with proteins, other molecules, and metal ions.

Optimal design strategies for customized ET probes are presented, as well as techniques for distance determination in protein-DNA complexes and the detection of topological DNA alterations, mutations, DNA breaks and single nucleotide polymorphisms. Merging work on nanotechnology, and fluorescent probes, authors describe in detail the design and application of ET-using molecular devices, such as biosensors, molecular machines, and logic gates for molecular scale computation.

Fluorescent Energy Transfer Nucleic Acid Probes

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